Transmission Electron Microscopy (TEM) Imaging

Hi-Vac SEM
Low-Vac SEM
Cryo SEM
TEM Imaging

Transmission electron microscopy (TEM) is useful in viewing biological samples requiring information from the sub-cellular level, hydrated particles such as microbes and viruses, and biochemical isolates such as protein and membrane fractions.  Numerous materials samples such as nano-particles, thin film components, and crystals are easily viewed using TEM when the size limits use of other imaging tools. However, regardless of the sample, the most critical factor is proper sample preparation in order to get the very best images and thus the information desired.

Biological sample preparation requires substantial knowledge and experience to determine the appropriate preparation methods to best secure the desired information. It may be as straightforward as routine chemical fixation, embedding, and thin sectioning. There may be special orientation needs, desire to locate specific proteins using immunocytochemical techniques, or cryo-preparation utilizing high pressure freezing and freeze substitution techniques.

Transmission Electron Microscopy (TEM)

Contact us and we can help you devise a protocol that will best suit your sample and elucidate the desired information from it.

Negative staining, utilizing heavy elements such as uranyl acetate or phosphotungstic acid, is routinely used for imaging isolated particles. Viruses, protein molecules and many inorganic samples, such as polymer particles and carbon nanotubes, are typical samples imaged using negative staining. We also do routine imaging of nanoparticles to document size and shape information, as well as sample uniformity.